Anti-neuronal antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis
© Springer-Verlag 2008
Received: 5 July 2008
Accepted: 24 July 2008
Published: 12 August 2008
Infectious-immunogenic mechanisms have been proposed for the pathogenesis of the syndrome of transient headache and neurological deficits with cerebrospinal fluid lymphocytosis (HaNDL) . To investigate this assumption, we performed immunohistochemistry and Western blot studies with sera and cerebrospinal fluid (CSF) of two HaNDL patients. Serum and CSF IgGs of both patients showed strong reactivity with the nuclei of rat central nervous system (CNS) cells and proteins extracted from neuroblastoma cells. This reactivity was not observed with samples of a sporadic hemiplegic migraine (SHM) patient and 12 migraine patients used as controls. The CNS binding pattern of HaNDL IgG was easily distinguishable from that of other well-known anti-neuronal nuclear antibodies (ANNA) such as anti-Hu, anti-Ri, and anti-nuclear antibody (ANA). The presence of an anti-neuronal immune response may support the autoimmune hypothesis as a pathogenic factor for HaNDL and should be further investigated in larger series.
To show the presence of a possible autoimmune response in this syndrome, sera and CSF of two HaNDL patients were investigated for anti-neuronal antibodies. Serum/CSF of a SHM patient, sera from 12 migraine, 2 anti-Hu, 2 anti-Ri, 34 ANA positive patients and 30 healthy individuals were used as controls. All headache patients fulfilled the clinical criteria established by The International Headache Society .
Transient headache and neurological deficits with cerebrospinal fluid lymphocytosis patients were 35- (H1) and 38-year-old (H2) men presenting with three episodes of throbbing headache, dysphasia/dysarthria, marching right hemiparesis and hemihypoesthesia (H1 only) lasting for 2–9 h. Their CSF revealed increased lymphocytes [496/mm3 (H1) and 55/mm3 (H2)] and normal (H1) or increased (H2) protein content (144 mg/dl). A survey for viral aseptic meningoencephalitis yielded negative results for both patients. H1 had normal cranial MRI and EEG examinations and H2 had left fronto-parietal leptomeningeal enhancement on MRI, left temporal and parietal lobe hypoperfusion on SPECT and slowing of the left fronto-temporal activity on EEG. Both patients recovered completely with flunarizine, lamotrigine or acetylsalicylic acid. CSF samples obtained 3 months after first presentation were normal.
All patients and control sera were tested with immunohistochemistry performed with frozen rat brain sections and Western blot performed with SY5Y neuroblastoma cells separated by electrophoresis on a 4–12% polyacrylamide gel, as described previously . Reactivity of the samples with the non-neuronal tissues was assessed with a kit containing primate intestine and HEp-2 cells (Euroimmun, Lübeck, Germany).
Autoimmunity has long been proposed as a possible pathogenic factor for HaNDL . The identification of novel anti-neuronal antibodies primarily directed against the nuclei of cerebral and cerebellar Purkinje cells in the sera and CSF of both HaNDL patients supports the presence of an immune response against CNS neurons and implies an abnormal humoral autoimmune reactivity in the pathogenesis of HaNDL.
Conflict of interest
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