Association between Chlamydia pneumoniae IgG antibodies and migraine
© Springer-Verlag 2009
Received: 5 November 2008
Accepted: 6 January 2009
Published: 24 February 2009
In this study, there is a confirmed association between cerebral infarction with migraine and Chlamydia pneumoniae infection, but the association between C. pneumoniae IgG antibodies and migraine in the general population has not been investigated. C. pneumoniae IgG antibody levels were compared in 329 adult Chinese patients, who met the International Classification of Headache Disorders 2nd Edition (ICHD II) criteria for migraine, and in 329 healthy subjects. Factors such as gender, age, smoking, consumption of pickle, and body mass index were evaluated. One hundred and ninety-five (59.2%) migraine sufferers and 70 (21.27%) controls were C. pneumoniae IgG antibody-seropositive (P < 0.05). Based on a multivariate stepwise logistic model, the odds’ ratios for C. pneumoniae IgG antibody seropositivity, body mass index, smoking, and consumption of pickle were 3.397 (P = 0.000), 0.858 (P = 0.014), 1.692 (P = 0.012), and 5.469 (P = 0.0000), respectively. In conclusion, C. pneumoniae IgG antibodies may be a risk factor for migraine.
KeywordsChlamydia pneumoniae IgG antibody Migraine Immunoglobulin
Migraine is a common type of headache with specific characteristics, including unilaterality, throbbing pain, photophobia or phonophobia, and nausea or vomiting . Several large-scale epidemiological studies have revealed that the prevalence of migraine ranges from 6 to 13% in the general population [2–6].
Migraine is a recurrent neurovascular headache, but its mechanism is still unclear; it may be influenced by genetic, endocrine, neurological or immune mechanisms, as well as by other relevant factors. Headaches, in particular migraine, are known to be independent risk factors for ischemic stroke [4–13]. The association between migraine and stroke is more prominent in young women, particularly, in those taking oral contraceptives [8–11]. However, in middle aged and elderly adults of either sex, the association is controversial. Several epidemiological studies have indicated that severe headache and migraine should be considered as risk factors for future stroke, prior to the age of 70 [5, 12].
Chlamydia pneumoniae is an important pathogen in infections of the respiratory tract  and numerous reports have recently suggested its association with atherosclerosis. Infection of the vascular wall with C. pneumoniae has been linked to ischemic heart disease [14–17] and stroke [18–24] in epidemiological studies as well as in pathological studies using immunocytochemistry, polymerase chain reaction (PCR), and electron microscopy. In Japan, Miyashita et al.  reported a significant elevation in C. pneumoniae antibody levels in a patient with acute myocardial infarction and Kawamoto et al.  showed a significant relation between atherosclerotic lesions and common carotid arteries. Kawashima and Kawada  demonstrated a significant elevation of C. pneumoniae antibody levels in a patient with acute ischemic stroke.
A correlation between cerebral infarction with migraine and C. pneumoniae infection has been confirmed, but a correlation between C. pneumoniae IgG antibody levels and migraine has not been investigated in the general population. We therefore investigated this relationship between C. pneumoniae IgG antibodies and migraine.
Subjects and methods
Demographic characteristics of migraine and control subjects
Control (N = 329)
Migraine (N = 329)
Gender, male, N
29.2 ± 8.2
31.2 ± 9.2
Smoking, N (over 5 years, above 15 packs/month)
Pickle, N (over 3 years, above 30 g/day)
Body mass index (kg/m2)
23.82 ± 1.56
23.81 ± 1.91
All blood samples were taken and separated in the morning.
Infection was determined by measuring anti-C. pneumoniae IgG-specific antibody levels (Cp IgG index) using an enzyme-linked immunosorbent assay method (“HITAZYME C. pneumoniae”, Hitachi Chemical Co. Ltd., Tokyo, Japan). Levels were measured in serum from fasting blood, which had been preserved at −70°C. Levels below the 1.10 Cp IgG index were classified as seronegative and those above 1.10 as seropositive .
The results are shown as mean value ± standard deviation. The results were analyzed statistically using SPSS 10.0J (Statistical Package for Social Science Inc., Chicago, IL, USA) and compared using χ2 tests. A value of P < 0.05 was considered significant. The relationships between the presence of migraine and a positive Cp IgG index, gender (female 0; male 1), age, body mass index (BMI: body weight/height2), smoking, and consumption of pickle were examined by multiple logistic regression analysis.
Chlamydia pneumoniae IgG antibodies in patients and controls
Seropositivity rates (%)
Relation between migraine and C. pneumoniae IgG antibodies as determined by multiple regression analysis logistic
Body mass index (kg/m2)
Smoking, N (%) (>5 years, >15 packs/month)
Pickle (>3 years, >30 g/day)
C. pneumoniae IgG
Migraine is a debilitating recurrent primary headache disorder, but its pathogenesis is still unclear. Migraine headaches have a complex pathophysiology and both vascular and neural theories have been proposed. One hypothesis suggests that the process begins with a series of destabilizing events within the brain that trigger cortical spreading depression (CSD) . CSD can cause both migraine aura and trigeminal activation, which, in turn, promotes neuropeptide release and triggers peripheral and central mechanisms that promote headache and autonomic activation. Susceptibility to CSD and to migraine is, in part, genetically determined. The best evidence to date comes from certain subtypes of migraine with aura, in which, point mutations in genes controlling the translocation of calcium, sodium and potassium have been implicated. When the dura mater cranial vascular wall by stimulating the trigeminal nerve endings, vasoactive peptide substances such as substance P, calcitonin gene-related peptide, and neurokinin are released [29, 30], causing neurogenic inflammation. Even minor chemical or mechanical stimulation of the sensitized trigeminal nerve also causes excessive excitement. Injury receptors at trigeminal nerve endings can stimulate the trigeminal nerve, thereby causing headache, activating the autonomic nervous system, and giving rise to symptoms such as nausea and vomiting. Trigeminal nerve inflammation and allergic trigeminal nerve provide a theoretical basis for migraine [31, 32]. C. pneumoniae IgG antibody production following infection can lead to inflammation and sensitization of the trigeminal nerve, so leading to migraine attack.
The results of this study showed that 195 (59.2%) migraine patients and 70 (21.27%) controls were C. pneumoniae antibody-seropositive, while Nabipour et al. found a seropositivity rate of 37.7% in healthy humans. The discrepancy between these results could be related to differences in case selection, the number of cases, or to regional differences .
The results of this study suggest that there is a correlation between C. pneumoniae IgG antibodies and migraine. It is possible that C. pneumoniae IgG antibodies may damage the brain vasculature, promote atherosclerosis and disrupt endothelial cell function, thereby stimulating vasospasm and damaging the blood–brain barrier. Changes in the environment can also stimulate a migraine attack due to the inflammation and sensitization of the trigeminal nerve caused directly by C. pneumoniae IgG antibodies.
This work was supported by Hubei province health department.
Conflict of interest
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