The Journal of Headache and Pain

Official Journal of the "European Headache Federation" and of "Lifting The Burden - The Global Campaign against Headache"

The Journal of Headache and Pain Cover Image

Volume 15 Supplement 1

Abstracts from the 4th European Headache and Migraine Trust International Congress: EHMTIC 2014

Open Access

EHMTI-0219. Quantitative histological examinations of inter- and intraindividual differences in the dural vasculature of the mouse

  • B Mecheri1,
  • F Paris1 and
  • H Lübbert1
The Journal of Headache and Pain201415(Suppl 1):F19

Published: 18 September 2014


MigrainePulmonary HypertensionDura MatermCPPVascular Alteration


To examine both migraine and pulmonary hypertension (PH) we developed a hypoxic mouse model. Under hypoxic conditions (four weeks at 10% O2) mice develop typical symptoms of PH, characterized by an increased muscularization of arterial blood vessels in the lung. The vessel remodeling can be prevented by the chronic application of 5-HT2B receptor antagonists. We found that hypoxic mice also develop increased sensitivity towards the 5-HT2 receptor agonist meta-Chlorophenylpiperazine (mCPP). In contrast to the control group (normoxic mice kept at 20% O2) at low doses of 1 µg / kg body weight, mCPP triggers plasma protein extravasation (PPE) in the dura mater in hypoxic mice. Dural PPE is a quantifiable indicator of migraine-like events in animal models.


The sensitization towards mCPP may coincide with structural remodeling processes in arterial blood vessels of the dura mater, possibly exhibiting similarities to vascular alterations in the lung. A prerequisite in the investigation of vascular alterations in the dura mater is a thorough understanding of the normal organization of dural vasculature in mice and of the extent of its normal biological variance.


Histological examinations of the length of dural vessels were performed to quantify and statistically analyze inter- and intraindividual differences.


Interindividual differences: The arteriole total lengths are 15% far from the arithmetic mean.

Intraindividual differences: The difference in the length of arterioles represents 12% of the total length of the arterioles.


On the basis of these results examinations of dural vessel of hypoxic mice can be investigated.

No conflict of interest.

Authors’ Affiliations

Department of Animal Physiology, Faculty of Biology and Biotechnology, Bochum, Germany


© Mecheri et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.