The Journal of Headache and Pain

Official Journal of the "European Headache Federation" and of "Lifting The Burden - The Global Campaign against Headache"

The Journal of Headache and Pain Cover Image

Volume 15 Supplement 1

Abstracts from the 4th European Headache and Migraine Trust International Congress: EHMTIC 2014

Open Access

EHMTI-0308. Cortical spreading depression increases NR2A/NR2B ratio by altering numbers of nr2a and nr2b subunit-containing nmda receptors in the hippocampus

  • P Hansrivijit1,
  • S Vibulyaseck1,
  • M Maneepark2,
  • S Bongsebandhu-Phubhakdi1 and
  • A Srikiatkhachorn1
The Journal of Headache and Pain201415(Suppl 1):F8

Published: 18 September 2014


GlutamateMigraineWestern Blot AnalysisNMDANMDA Receptor


Cortical spreading depression (CSD), an underlying mechanism of migraine aura, that propagates to the hippocampus is believed to disrupt hippocampal metaplasticity owing to hippocampus-associated symptoms (e.g. amnesia) manifested by patients with chronic migraine. Our previous study showed that this aberration is mediated by AMPA receptors but roles of NMDA receptors are yet to be discovered.


To exhibit the alteration of NR2A/NR2B response ratio in terms of total numbers of individual NR2A and NR2B-subunits following CSD stimulation.


Adult Wistar rats were divided into CSD and control group for electrophysiological study (n = 6, each group) and Western blot analysis (n = 15, each group). Electrophysiological response of both NR2A and NR2B were recorded in terms of field-excitatory post-synaptic potentials (fEPSPs). The fEPSP of NR2A was divided by those of NR2B in both control and CSD groups. Western blot analysis was employed to quantify total numbers of hippocampal NR2A and NR2B.


NR2A/NR2B ratio of CSD group significantly increased in comparison with control group (p = 0.018). From Western blot analysis, intensity of NR2A component was significantly elevated (p = 0.048) whilst that of NR2B was diminished (p = 0.002).


As numerous studies demonstrated that increased hippocampal NR2A/NR2B ratio is linked to impaired long-term potentiation (LTP), our research adds that increase in NR2A/NR2B ratio following CSD stimulation is possibly due to transcriptional up/down-regulation of NR2A and NR2B, respectively. Combining with our previous study, we conclude that CSD impairs memory processes by disrupting both glutamate AMPA and NMDA receptors.

No conflict of interest.

Authors’ Affiliations

Physiology, Faculty of Medicine Chulalongkorn University, Bangkok, Thailand
Physiology, Faculty of Science Srinakharinwirot University, Bangkok, Thailand


© Hansrivijit et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.