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The Journal of Headache and Pain

Official Journal of the "European Headache Federation" and of "Lifting The Burden - The Global Campaign against Headache"

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Volume 16 Supplement 1

1st Joint ANIRCEF-SISC Congress

Open Access

P013. 25(OH)D Level and headache in children sample

  • Elisabetta Tozzi1Email author,
  • Alessandra Boncristiano2,
  • Annarita Antenucci2,
  • Simona Di Loreto2 and
  • Giovanni Farello2
The Journal of Headache and Pain201516(Suppl 1):A84

Published: 28 September 2015


MigraineSerum LevelSerum ConcentrationBlood LevelClinical Parameter


Statistical significance between serum 25(OH)D levels and headache in the pediatric population is still controversial. However, a supplementary vitamin D therapy in children with headache allowed us to note an improvement in the clinical symptoms in terms of frequency and severity[1].


To study vitamin D blood levels in primary headaches and to show the relationship with clinical parameters influencing clinical course of the headache.

Materials and methods

Sixty-seven males (42%) and 92 females (58%), aged between 5 to 18 years, suffering from headache, were divided into three diagnostic categories, according to the ICHD-III classification: migraine with aura (MWA), migraine without aura (MWoA) and tension-type headache (TTH). Serum vitamin D level lower than 20 ng/ml was considered pathological. Immunohistochemical methods of chemiluminescence were used to determine blood 25(OH)D level.


Ninety-one patients (57%) received a diagnosis of MWoA, 32 (20%) of MWA and 36 (23%) of TTH. Hypovitaminosis D was found in 56% of the children with MWoA, in 50% of patients with MWA and in 44.4% with TTH. Twelve percent of all the sample showed severe Hypovitaminosis D (<10 ng/ml). There were neither statistically significant differences comparing vitamin D serum levels between males and females in each category of headache (p = 0.36), nor more severe deficiency of vitamin D in overweight children with BMI>90°C (p = 0.47), and serum concentration was not lower in adolescents and pre-adolescent than children under 10 years. A difference resulted in the limits of statistical significance (p = 0.07) in 25(OH)D serum level in children with MWoA compared to patients with MWA and THH.


Our study shows a high incidence of vitamin D deficiency in the pediatric population with headache (56%), particularly in migraineurs without aura[2]. Probably this deficiency could be directly correlated with a higher frequency of migraine attacks in children with MWoA.

Written informed consent to publication was obtained from the patient(s).



We thank Professor Stefano Necozione for the statistic analysis.

Authors’ Affiliations

Neuropsychiatric Clinic, Hospital of L'Aquila, Department of life, health and environmental sciences, University L'Aquila, L'Aquila, Italy
Pediatric Clinic, Hospital of L'Aquila, L'Aquila, Italy


  1. Cayir A, et al: Effect of vitamin D in addition to amitriptyline on migraine attacks in pediatric patients. Braz J Med Biol Res. 2014, 47: 349-54. 10.1590/1414-431X20143606.PubMed CentralView ArticlePubMedGoogle Scholar
  2. Mottaghi T, et al: The relationship between serum levels of vitamin D and migraine. J Res Med Sci. 2013, 18: S66-70.PubMed CentralPubMedGoogle Scholar


© Tozzi et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.


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